The effect of short-term intensive insulin therapy on circulating T cell subpopulations in patients with newly diagnosed type 2 diabetes mellitus

  • Author Footnotes
    1 These authors contributed equally to this study.
    Liang Cheng
    Footnotes
    1 These authors contributed equally to this study.
    Affiliations
    Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, China

    Department of Endocrinology, Huai’an Second People’s Hospital and the Affiliated Huai’an Hospital of Xuzhou Medical University, Huaian, Jiangsu, China
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  • Author Footnotes
    1 These authors contributed equally to this study.
    Fan Yang
    Footnotes
    1 These authors contributed equally to this study.
    Affiliations
    Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, China

    Department of Endocrinology, Yancheng City No. 1 People’s Hospital, Yancheng, Jiangsu, China
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  • Xin Cao
    Affiliations
    Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, China
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  • Guo-Qing Li
    Affiliations
    Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, China
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  • Ting-Ting Lu
    Affiliations
    Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, China
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  • Yun-Qing Zhu
    Affiliations
    Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, China
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  • Yun Hu
    Correspondence
    Corresponding authors at: Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, ChangLe Street 68, Nanjing 210002, China.
    Affiliations
    Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, China
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  • Xiao-Ming Mao
    Correspondence
    Corresponding authors at: Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, ChangLe Street 68, Nanjing 210002, China.
    Affiliations
    Department of Endocrinology, the Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, China
    Search for articles by this author
  • Author Footnotes
    1 These authors contributed equally to this study.
Published:February 10, 2019DOI:https://doi.org/10.1016/j.diabres.2019.02.007

      Abstract

      Aims

      To evaluate the effect of short-term intensive insulin therapy on circulating T cell subpopulations in patients with newly diagnosed type 2 diabetes mellitus (T2DM).

      Methods

      A total of 113 patients with T2DM and 28 normal subjects were enrolled. Demographic parameters and biochemical markers were collected at baseline, and flow cytometry was applied to determine the proportion of T cell subpopulations in participants. Then the patients underwent continuous subcutaneous insulin injection (CSII) treatment with euglycemia for 2 weeks, and the T cell subpopulations were measured again after CSII treatment.

      Results

      Compared with normal subjects, the proportion of Th1 cells and the ratio of Th1/Th2 increased, the proportion of Treg cells decreased in patients with T2DM (p < 0.05 for all). The ratio of Th1/Th2 was positively correlated with glycosylated hemoglobin A1c (HbA1c) and negatively correlated with high density lipoprotein cholesterol (HDL-C). Furthermore, there were negative associations between the proportion of Treg cells and fasting plasma glucose, HbA1c, triglyceride, low density lipoprotein cholesterol, and positive association between the proportion of Treg cells and HDL-C. After CSII treatment, the proportion of Th1 cells and the ratio of Th1/Th2 decreased (p < 0.05 for both), the proportion of Treg cells increased in patients with T2DM (p < 0.05).

      Conclusions

      Short-term intensive insulin therapy could modulate circulating T cell subpopulations in patients with T2DM, which might alleviate inflammatory responses caused by hyperglycemia.
      This study was registered with ChiCTR-OPN-17010405.

      Keywords

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