Highlights
- •Cross-sectional study of telomeres in Type 1 diabetes and non-diabetic subjects.
- •Shorter telomeres in T1D vs. non-diabetic subjects.
- •Relative telomere length (RTL) do not correlate with HbA1c, oxidation or smoking.
- •RTL correlates inversely with age, T1D duration, inflammation and vascular function.
Abstract
Objective
To determine if white blood cell (WBC) telomeres are shorter in Type 1 diabetes (T1D)
than in subjects without diabetes (non-DB), and shorter in T1D subjects with vs. without
vascular complications; and to determine associations with vascular biomarkers.
Research design and methods
WBC relative telomere length (RTL) was determined by quantitative PCR in a cross-sectional
study of 140 non-DB and 199 T1D adults, including 128 subjects without vascular complications
(T1DNoCx) and 71 subjects with vascular complications (T1DCx). Relationships of RTL
with age, T1D duration, arterial elasticity, pulse pressure and vascular risk factors
were determined.
Results
RTL did not differ by gender within T1D and non-DB groups. Age-adjusted RTL was shorter
in T1D vs. non-DB subjects (1.48 ± 0.03 AU vs. 1.64 ± 0.04 AU, p = 0.002), but did not differ by T1D complication status (T1DNoCX 1.50 ± 0.04 vs. T1DCX 1.46 ± 0.05, p = 0.50), nor correlate with arterial elasticity. Univariate analysis in T1D showed RTL
correlated (inversely) with age r = −0.27, p = 0.0001, T1D duration r = −0.16, p = 0.03, and pulse pressure (r = −0.15, p = 0.04), but not with HbA1c, BP, renal function (serum creatinine, ACR, eGFR), lipids,
insulin sensitivity, inflammation (CRP, CAMs) or oxidative stress (OxLDL, OxLDL/LDL-C,
MPO, PON-1). Multiple regression analysis showed independent determinants of RTL were
age and T1D presence (r = 0.29, p < 0.0001).
Conclusions
In this cross-sectional study telomeres were shorter in T1D. RTL correlated inversely
with T1D duration, but did not differ by complication status and weakly correlated
with pulse pressure and vascular risk factors. Only age and T1D were independent determinants
of RTL. Longitudinal studies are merited.
Keywords
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Article info
Publication history
Published online: April 28, 2016
Accepted:
April 21,
2016
Received in revised form:
March 25,
2016
Received:
January 5,
2016
Identification
Copyright
© 2016 Elsevier Ireland Ltd. All rights reserved.
