Thymosin beta 4 ameliorates hyperglycemia and improves insulin resistance of KK Cg-Ay/J mouse

Published:January 05, 2012DOI:https://doi.org/10.1016/j.diabres.2011.12.009

      Abstract

      Object

      To evaluate the efficacy of thymosin beta 4 (Tβ4) on hyperglycemia and insulin sensitivity in a mouse model of type 2 diabetes mellitus (T2DM).

      Methods

      KK mice were divided into the following groups: KK control group, with saline treatment; KK Tβ4 group, with daily Tβ4 100 ng/10 g body weight intraperitoneal injection for 12 weeks. Non-diabetic C57BL mice were used as normal control. OGTT, plasma insulin, HbA1c, serum adiponectin, Tβ4, cholesterol, and triglyceride were measured before and after Tβ4 treatment. The phosphorylated AKT and total AKT protein levels of skeletal muscle from all groups were determined.

      Results

      After Tβ4 treatment, repeat OGTT showed a significant decrease in glucose profiles in the KK Tβ4 group compared with the KK control group. The KK-Tβ4 group had reduced mean HbA1c and triglyceride levels, and increased adiponectin compared with KK control group. C57BL mice showed normal glucose homeostasis. The phosphorylated AKT levels of skeletal muscle were significantly increased in KK Tβ4 group compared with KK control group after glucose stimulation. C57BL mice showed no changes in phosphorylated AKT levels after Tβ4 treatment.

      Conclusions

      4 improved glucose intolerance and ameliorated insulin resistance in KK mouse. Tβ4 may be a potential alternative insulin sensitizer for treatment of T2DM.

      Keywords

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