Abstract
Aims
To clarify the levels of protein oxidation markers such as protein carbonyl (PCO),
protein hydroperoxides (P-OOH), advanced oxidation protein products (AOPP) and nitrotyrosine
(NT), as well as antioxidative enzymes such as paraoxonase (PON-1) in women with and
without gestational diabetes mellitus (GDM).
Methods
The study was conducted on 23 women with GDM and 22 women without GDM. The levels
of the P-OOH, AOPP, and PON-1 were determined by colorimetric methods; whereas NT
and PCO levels were measured by ELISA.
Results
The concentrations of protein oxidation markers were significantly increased and PON1
activity was significantly decreased in GDM group compared to those of normal pregnant
women. The control group showed a significant negative correlation between PON-1 and
PCO (r = −0.451, p = 0.027); whereas in GDM group, there was a significant positive correlation between
P-OOH and HbA1c (r = 0.89, p = 0.001). There was no significant correlation between AOPP, PON-1, P-OOH, PCO, and
HbA1c in either group.
Conclusions
There is evidence of a possible association between protein oxidation and decreased
PON1 activity in GDM. The increase in protein oxidation parameters in the GDM group
leading to decreased PON1 activity might, we think, create a predisposition for clinical
complications in GDM group.
Keywords
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Article info
Publication history
Published online: September 01, 2011
Accepted:
August 2,
2011
Received in revised form:
July 28,
2011
Received:
July 1,
2011
Identification
Copyright
© 2011 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
