Abstract
Aim
In this study, we examined changes in asymmetric dimethylarginine (ADMA), dimethylarginine
dimethylaminohydrolase (DDAH), nitric oxide synthesis (NOS), and the arginine methylation
of organ proteins during the development of diabetes in mice.
Methods
Db/db mice developed significant obesity and fasting hyperglycemia during diabetogenesis.
During diabetogenesis, the expression of ADMA and nNOS was increased, while that of
DDAH1 and protein-arginine methyltransferase 1 (PRMT1) was decreased. Additionally,
arginine methylation in the liver and adipose tissue was altered during diabetogenesis.
Results
Changes were evident at 75, 60, and 52 kDa in liver tissue and at 38 and 25 kDa in adipose tissue. Collectively, DDAH and ADMA are closely associated with the
development of obesity and diabetes, and the arginine methylation levels of certain
proteins were changed during diabetes development.
Conclusion
Protein arginine methylation plays a role in the pathogenesis of diabetes.
Keywords
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Article info
Publication history
Published online: August 22, 2011
Accepted:
July 4,
2011
Received in revised form:
June 23,
2011
Received:
March 21,
2011
Identification
Copyright
© 2011 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
