Broccoli sprouts powder could improve serum triglyceride and oxidized LDL/LDL-cholesterol ratio in type 2 diabetic patients: A randomized double-blind placebo-controlled clinical trial

  • Author Footnotes
    1 Address: No. 24 Parvaneh St, Yemen St, Chamran Exp, Tehran 19395-4763, Iran.
    Zahra Bahadoran
    Footnotes
    1 Address: No. 24 Parvaneh St, Yemen St, Chamran Exp, Tehran 19395-4763, Iran.
    Affiliations
    Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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  • Author Footnotes
    2 Address: No. 46 Arghavan-e-gharbi St, Farahzadi Blv, Shahrak-e-Ghods. Tehran 19395-4741, Iran.
    Parvin Mirmiran
    Footnotes
    2 Address: No. 46 Arghavan-e-gharbi St, Farahzadi Blv, Shahrak-e-Ghods. Tehran 19395-4741, Iran.
    Affiliations
    Department of Clinical Nutrition and Dietetics, Faculty of Nutrition Sciences and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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  • Farhad Hosseinpanah
    Correspondence
    Corresponding author at: No. 24 Parvaneh St, Yemen St, Chamran Exp, Tehran 19395-4763, Iran. Tel.: +98 21 224 32 500; fax: +98 21 224 16 264/02 463.
    Affiliations
    Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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  • Author Footnotes
    3 Address: No. 46 Ramin-Malakuti St, Patris Lumumba St, Sattar-khan St, Tehran 14155-3316, Iran.
    Asadolah Rajab
    Footnotes
    3 Address: No. 46 Ramin-Malakuti St, Patris Lumumba St, Sattar-khan St, Tehran 14155-3316, Iran.
    Affiliations
    Iran Diabetes Society, Tehran, Iran
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  • Author Footnotes
    1 Address: No. 24 Parvaneh St, Yemen St, Chamran Exp, Tehran 19395-4763, Iran.
    Golale Asghari
    Footnotes
    1 Address: No. 24 Parvaneh St, Yemen St, Chamran Exp, Tehran 19395-4763, Iran.
    Affiliations
    Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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  • Author Footnotes
    1 Address: No. 24 Parvaneh St, Yemen St, Chamran Exp, Tehran 19395-4763, Iran.
    Fereidoun Azizi
    Footnotes
    1 Address: No. 24 Parvaneh St, Yemen St, Chamran Exp, Tehran 19395-4763, Iran.
    Affiliations
    Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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  • Author Footnotes
    1 Address: No. 24 Parvaneh St, Yemen St, Chamran Exp, Tehran 19395-4763, Iran.
    2 Address: No. 46 Arghavan-e-gharbi St, Farahzadi Blv, Shahrak-e-Ghods. Tehran 19395-4741, Iran.
    3 Address: No. 46 Ramin-Malakuti St, Patris Lumumba St, Sattar-khan St, Tehran 14155-3316, Iran.
Published:February 13, 2012DOI:https://doi.org/10.1016/j.diabres.2012.01.009

      Abstract

      Background and aims

      In this study, broccoli sprout powder (BSP), a good source of bioactive components, was used as supplementary treatment in type 2 diabetic patients.

      Methods

      This randomized clinical trial included 81 patients with type 2 diabetes. Participants were randomly assigned to consume 10 g/d BSP (group A), 5 g/d BSP (group B), or the placebo (group C), each for 4 weeks. Fasting blood glucose (FBS), total cholesterol (TC), triglyceride concentration (TG), LDL-C, HDL-C, and oxidized-LDL were measured at baseline and 4 weeks after treatment. The ratios of OX-LDL/LDL, atherogenic index of plasma (AIP; log TG/HDL), TC/HDL and LDL/HDL were calculated as cardiovascular risk factors parameters, at baseline and 4 weeks after treatment.

      Results

      Seventy-two patients completed the study; n = 23, 26 and 23 for groups A, B and C, respectively. After 4 weeks, BSP in dose of 10 g/d, significantly decreased serum triglycerides, OX-LDL/LDL ratio and AIP (p < 0.05 for treatment effect). HDL-C concentration was significantly higher (p < 0.01 for treatment) in group A as compared with group B and placebo.

      Conclusions

      BSP as supplementary treatment in type 2 diabetes could have favorable effects on lipid profiles and OX-LDL/LDL ratio, as risk factors for cardiovascular disease.

      Keywords

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      References

        • Gadi R.
        • Samaha F.F.
        Dyslipidemia in type 2 diabetes mellitus.
        Curr Diab Rep. 2007; 7: 228-234
        • Taskinen M.R.
        Type 2 diabetes as a lipid disorder.
        Curr Mol Med. 2005; 5: 297-308
        • Santaguida P.L.
        • Balion C.
        • Hunt D.
        Diagnosis, prognosis, and treatment of impaired glucose tolerance and impaired fasting glucose.
        Evid Rep Technol Assess. 2008; 12: 1-11
        • Davi G.
        • Sanitilli F.
        • Patrono C.
        Nutraceutical in diabetes and metabolic syndrome.
        Cardiovasc Ther. 2010; 28: 216-226
        • Dembinska-Kiec A.
        • Mykkänen O.
        • Kiec-Wilk B.
        • Mykkänen H.
        Antioxidant phytochemicals against type 2 diabetes.
        Br J Nutr. 2008; 99: ES109-ES117
        • Jeffery E.
        • Araya M.
        Physiological effects of broccoli consumption.
        Phytochem Rev. 2009; 8: 283-298
        • Keum Y.
        • Jeong W.
        • Kong T.
        Chemoprevention by isothiocyanates and underlying molecular signaling mechanisms.
        Mutat Res. 2004; 555: 191-192
        • Verkerk R.
        • Schreiner M.
        • Krumbein A.
        • Ciska E.
        • Holst B.
        • Rowland I.
        • et al.
        Glucosinolates in brassica vegetable: the influence of the food supply chain on intake, bioavailability and human health.
        Mol Nutr Food Res. 2009; 53: S219-S266
        • Lee J.
        • Shin H.
        • Lee Y.
        • Kim A.
        • Lee M.
        Effect of broccoli sprouts on cholesterol-lowering and anti-obesity effects in rats fed high fat diet.
        J Korean Soc Food Sci Nutr. 2009; 38: 309-318
        • Moreno D.A.
        • Carvajal M.
        • López-Berenguer C.
        • García-Viguera C.
        Chemical and biological characterisation of nutraceutical compounds of broccoli.
        J Pharm Biochem Anal. 2006; 41: 1508-1522
        • Riedl M.A.
        • Saxon A.
        • Diaz-Sanchez D.
        Oral sulforaphane increases phase II antioxidant enzymes in the human upper airway.
        Clin Immunol. 2009; 130: 244-251
        • Fahey J.
        • Talalay P.
        Antioxidant function of sulforaphane: a potent inducer of phase 2 detoxyfication enzymes.
        Food Chem Toxicol. 1999; 37: 973-979
        • Murashima M.
        • Watanabe S.
        • Zhuo X.G.
        • Uehara M.
        • Kurashige A.
        Phase 1 study of multiple biomarkers for metabolism and oxidative stress after one-week intake of broccoli sprouts.
        Biofactors. 2004; 22: 271-275
        • Schulz K.F.
        • Altman D.G.
        • Moher D.
        CONSORT 2010 statement. Updated guidelines for reporting parallel group randomized trials.
        J Clin Epidemiol. 2010; 63: 834-840
      1. The Nutrient Data Laboratory. Food Composition Table (FCT), Food and Nutrition Information Center, United State Department of Agriculture (USDA). www.nal.usda.gov/fnic/foodcomp.

        • Azar M.
        • Sarkisian E.
        Food composition table of Iran. National Nutrition and Food Research Institute.
        Shahid Beheshti University Press, 1980
        • Cordova C.M.
        • Schneider C.R.
        • Juttle I.D.
        • Cordova M.M.
        Comparison of LDL-cholesterol direct measurement with the estimate using the friedewald formula in a sample of 10664 patients.
        Arq Bras Cardiol. 2004; 83: 482-487
        • Sarwar N.
        • Danesh J.
        • Eiriksdottir G.
        • Sigurdsson G.
        • Wareham N.
        • Bingham S.
        • et al.
        Triglycerides and the risk of coronary heart disease: 10,158 incident cases among 262,525 participants in 29 Western prospective studies.
        Circulation. 2007; 115: 450-458
        • Hokanson J.E.
        • Austin M.A.
        Plasma triglyceride level is a risk factor for cardiovascular disease independent of high-density lipoprotein cholesterol level: a meta-analysis of population-based prospective studies.
        J Cardiovasc Risk. 1996; 3: 213-219
        • Nicolucci A.
        • De Berardis G.
        • Sacco M.
        • Tognoni G.
        Primary prevention of cardiovascular diseases in people with diabetes mellitus: a scientific statement from the American Heart Association and the American Diabetes Association.
        Diabetes Care. 2007; 30: 162-172
        • Jialal I.
        • Amess W.
        • Kaur M.
        Management of hypertriglyceridemia in the diabetic patient.
        Curr Diab Rep. 2010; 10: 316-320
        • Birjmohun R.S.
        • Hutten B.A.
        • Kastelein J.J.
        • Stroes E.S.
        Efficacy and safety of high-density lipoprotein cholesterol-increasing compounds: a meta-analysis of randomized controlled trials.
        J Am Coll Cardiol. 2005; 45: 185-197
        • Colhoun H.M.
        • Betteridge D.J.
        • Durrington P.N.
        • Hitman G.A.
        • Neil H.A.
        • Livingstone S.J.
        • et al.
        Primary prevention of cardiovascular disease with atorvastatin in type 2 diabetes in the Collaborative Atorvastatin Diabetes Study (CARDS): multicentre randomised placebo-controlled trial.
        Lancet. 2004; 364: 685-696
        • Shimada K.
        • Mokuno H.
        • Matsunaga E.
        • Miyazaki T.
        • Sumiyoshi K.
        • Kume A.
        • et al.
        Predictive value of circulating oxidized LDL for cardiac events in type 2 diabetic patients with coronary artery disease.
        Diabetes Care. 2004; 27: 843-844
        • Galland F.
        • Duvillard L.
        • Petit J.M.
        • Lagrost L.
        • Vaillant G.
        • Brun J.M.
        • et al.
        Effect of insulin treatment on plasma oxidized LDL/LDL-cholesterol ratio in type 2 diabetic patients.
        Diabetes Metab. 2006; 32: 625-631
        • Dobiasova M.
        • Frohlich J.
        The plasma parameter log (TG/HDL-C) as an atherogenic index: correlation with lipoprotein particle size and esterification rate in apo-B-lipoprotein-depleted plasma.
        Clin Biochem. 2001; 34: 583-588
        • Frohilich J.
        • Dobiasova M.
        Fractional esterification rate of cholesterol and ratio of triglyceride to HDL-C are powerful predictors of positive finding on coronary angiography.
        Clin Chem. 2003; 49: 1873-1880
        • Nair D.
        • Carrigan T.P.
        • Curtin R.J.
        • Popovic Z.B.
        • Kuzmiak S.
        • Schoenhagen P.
        • et al.
        Association of total cholesterol/high-density lipoprotein cholesterol ratio with proximal coronary atherosclerosis detected by multislice computed tomography.
        Prev Cardiol. 2009; 12: 19-26
        • Christiansen B.
        • Bellostas Muguerza N.
        • Petersen A.M.
        • Kveiborg B.
        • Madsen C.R.
        • Thomas H.
        • et al.
        Ingestion of broccoli sprouts does not improve endothelial function in humans with hypertension.
        PLoS One. 2010; 5: e12461
        • Kahlon T.S.
        • Chapman M.H.
        • Smith G.E.
        In vitro binding of bile acids by spinach, kale, brusseles sprouts, broccoli, mustard green, green bell pepper, cabbage and collards.
        Food Chem. 2007; 100: 1531-1536
        • Dunn S.E.
        • LeBlanc G.A.
        Hypocholesterolemic properties of plant indoles. Inhibition of acyl-CoA:cholesterol acyltransferase activity and reduction of serum LDL/VLDL cholesterol levels by glucobrassicin derivatives.
        Biochem Pharmacol. 1994; 47: 359-364
        • LeBlanc G.A.
        • Stuart J.D.
        • Dunn S.E.
        • Baldwin W.S.
        Effect of the plant compound indole-3-carbinol on hepatic cholesterol homoeostasis.
        Food Chem Toxicol. 1994; 32: 633-639
        • Maiyoh G.K.
        • Kuh J.E.
        • Casaschi A.
        • Theriault A.G.
        Cruciferous indole-3-carbinol inhibits apolipoprotein B secretion in HepG2 cells.
        J Nutr. 2007; 137: 2185-2189
        • Menke T.
        • Niklowitz P.
        • de Sousa G.
        • Reinehr T.
        • Andler W.
        Comparison of coenzyme Q10 plasma levels in obese and normal weight children.
        Clin Chim Acta. 2004; 349: 121-127
        • Wu L.
        • Noyan M.H.
        • Facci M.
        Dietary approach to attenuate oxidative stress, hypertension, and inflammation in the cardiovascular system.
        Proc Natl Acad Sci. 2004; 101: 7094-7099
        • Galan M.V.
        • Kishan A.A.
        • Silverman A.L.
        Oral broccoli sprouts for the treatment of Helicobacter pylori infection: a preliminary report.
        Dig Dis Sci. 2004; 49: 1088-1090