HLA-DQB1 genotypes and islet cell autoantibodies against GAD65 and IA-2 in relation to development of diabetes post partum in women with gestational diabetes mellitus

Papadopoulou, Anastasia; Lynch, Kristian F.; Anderberg, Eva; Landin-Olsson, Mona; Hansson, Ida; Agardh, Carl-David; Lernmark, Åke; Berntorp, Kerstin

doi:10.1016/j.diabres.2011.10.037

« Previous Next »Diabetes Research and Clinical Practice
Volume 95, Issue 2 , Pages 260-264, February 2012

HLA-DQB1 genotypes and islet cell autoantibodies against GAD65 and IA-2 in relation to development of diabetes post partum in women with gestational diabetes mellitus

Anastasia Papadopoulou
- Department of Clinical Sciences, Lund University, Malmö, Sweden
- Department of Endocrinology, Lund University, Skåne University Hospital, Malmö, Sweden
- Corresponding author at: Lund University/Clinical Research Centre, Department of Clinical Sciences, Entrance 72, Building 91, Level 10, SE-20502 Malmö, Sweden. Tel.: +46 701484005; fax: +46 40 391919.
Kristian F. Lynch
- Department of Clinical Sciences, Lund University, Malmö, Sweden
- Pediatrics Epidemiology Center, University of South Florida, Tampa, FL, USA
Eva Anderberg
- Department of Obstetrics and Gynaecology, Lund University, Skåne University Hospital, Lund, Sweden
Mona Landin-Olsson
- Department of Endocrinology, Lund University, Skåne University Hospital, Lund, Sweden
Ida Hansson
- Department of Clinical Sciences, Lund University, Malmö, Sweden
Carl-David Agardh
- Department of Clinical Sciences, Lund University, Malmö, Sweden
- Department of Endocrinology, Lund University, Skåne University Hospital, Malmö, Sweden
Åke Lernmark
- Department of Clinical Sciences, Lund University, Malmö, Sweden
Kerstin Berntorp
- Department of Endocrinology, Lund University, Skåne University Hospital, Malmö, Sweden

Received 13 October 2011; accepted 24 October 2011. published online 21 November 2011.

Abstract

Aims

To study HLA-DQB1 genes and islet cell autoantibodies against glutamic acid decarboxylase 65 (GADA) and insulinoma antigen-2 (IA-2A) in relation to diabetes post partum in mothers with diagnosed gestational diabetes mellitus (GDM).

Methods

During 2003–2004, women undergoing a 75g oral glucose tolerance test (OGTT) during pregnancy were invited to participate in the Mamma Study. Cut-off level defining GDM was a 2-h capillary blood glucose of 7.8mmol/L. 1–2 years after delivery a 75g OGTT was performed, GADA and IA-2A were measured and HLA-DQB1 genes analysed. Data were available for 452 mothers with previous GDM and 168 randomly selected control subjects.

Results

HLA-DQB1*0602 was negatively associated with GDM (p=0.033) and with development of diabetes post partum (p=0.017), whereas high risk HLA were not associated with GDM or with diabetes. The presence of GADA post partum was positively associated with diabetes post partum (p=0.0009), but not with impaired glucose tolerance.

Conclusions

Mothers with GDM and HLA-DQB1*0602 were less likely to develop diabetes after pregnancy, and type 1 diabetes associated high risk HLA genes did not predict type 1 diabetes post partum. Additionally, GADA were positively associated with diabetes development.

Abbreviations: BMI, Body mass index, CI, Confidence interval, HbA1c, Glycated hemoglobin, IA-2A, Insulinoma antigen-2 autoantibodies, IAA, Insulin autoantibodies, IGT, Impaired glucose tolerance, GADA, Glutamic acid decarboxylase 65 autoantibodies, GDM, Gestational diabetes mellitus, NGT, Normal glucose tolerance, OGTT, Oral glucose tolerance test, OR, Odds ratio, SD, Standard deviation, T1DM, Type 1 diabetes mellitus, WHO, World Health Organization