Acceleration of diabetic wound healing by collagen-binding vascular endothelial growth factor in diabetic rat model

Yan, Xin; Chen, Bing; Lin, Yue; Li, Yunjian; Xiao, Zhifeng; Hou, Xianglin; Tan, Qian; Dai, Jianwu

doi:10.1016/j.diabres.2010.07.001

« Previous Next »Diabetes Research and Clinical Practice
Volume 90, Issue 1 , Pages 66-72, October 2010

Acceleration of diabetic wound healing by collagen-binding vascular endothelial growth factor in diabetic rat model

Xin Yan
- Department of Burns and Plastic Surgery, Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China
- These authors contributed equally to this work.
Bing Chen
- Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, Beijing 100190, China
- These authors contributed equally to this work.
Yue Lin
- Department of Burns and Plastic Surgery, Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China
Yunjian Li
- Department of Burns and Plastic Surgery, Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China
Zhifeng Xiao
- Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, Beijing 100190, China
Xianglin Hou
- Yantai Zhenghai Biotechnology Company, Ltd, Yantai, Shangdong 264006, China
Qian Tan
- Department of Burns and Plastic Surgery, Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China
- Corresponding author. Tel.: +86 025 83107044; fax: +86 025 83107044.
Jianwu Dai
- Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, Beijing 100190, China
- Corresponding author. Tel.: +86 010 82614426; fax: +86 010 82614426.

Received 31 March 2010; received in revised form 20 June 2010; accepted 1 July 2010. published online 28 July 2010.

Abstract

Aims

Vascular endothelial growth factor (VEGF) is an important active protein for the induction of angiogenesis and plays an important role in the tissue regeneration of diabetic wounds. In this study, we used collagen-binding VEGF in a diabetic rat model to investigate the effects of this new method.

Methods

We produced a fusion protein (CBD-VEGF) consisting of VEGF and a collagen-binding domain (CBD), which allowed VEGF to bind to collagen. The diabetic rat models were made by injected streptozocin (STZ) peritoneally and removed full thickness skin on the back. All the rats were randomly divided into 3 groups: PBS group (n=24), NAT-VEGF group (n=24), and CBD-VEGF group (n=24). After model establishment, the dissolved drugs were evenly given on the wounds using syringe. The healing rates were calculated and compared among the groups and the tissues of the wound were taken and evaluated for histological analysis.

Results

The CBD-VEGF group showed better result in wound healing rate, better vascularization and higher amount of VEGF in the wound granulation tissue compared with NAT-VEGF group and control.

Conclusions

Topical application of CBD-VEGF can promote diabetic wound healing in rat model, which could potentially provide a better therapeutic option for diabetic wounds.

Keywords: VEGF, Wounds, Healing