Plasma apelin levels and apelin/APJ mRNA expression in patients with gestational diabetes mellitus

Telejko, Beata; Kuzmicki, Mariusz; Wawrusiewicz-Kurylonek, Natalia; Szamatowicz, Jacek; Nikolajuk, Agnieszka; Zonenberg, Anna; Zwierz-Gugala, Dorota; Jelski, Wojciech; Laudański, Piotr; Wilczynski, Jan; Kretowski, Adam; Gorska, Maria

doi:10.1016/j.diabres.2009.10.018

« Previous Next »Diabetes Research and Clinical Practice
Volume 87, Issue 2 , Pages 176-183, February 2010

Plasma apelin levels and apelin/APJ mRNA expression in patients with gestational diabetes mellitus☆

Beata Telejko
- Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, , Poland
- Corresponding author at: Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Białystok, M. Curie-Skłodowskiej 24A, 15-276 Białystok, Poland. Tel.: +48 857468239; fax: +48 857447611.
Mariusz Kuzmicki
- Department of Gynecology, Medical University of Bialystok, , Poland
Natalia Wawrusiewicz-Kurylonek
- Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, , Poland
Jacek Szamatowicz
- Department of Gynecology, Medical University of Bialystok, , Poland
Agnieszka Nikolajuk
- Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, , Poland
Anna Zonenberg
- Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, , Poland
Dorota Zwierz-Gugala
- Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, , Poland
Wojciech Jelski
- Department of Biochemical Diagnostics, Medical University of Bialystok, , Poland
Piotr Laudański
- Department of Gynecology, Medical University of Bialystok, , Poland
Jan Wilczynski
- Foetal-Maternal Medicine and Gynecology Department, Research Institute Polish Mother's Memorial Hospital (RI PMMH), Lodz, Poland
Adam Kretowski
- Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, , Poland
Maria Gorska
- Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, , Poland

Received 10 June 2009; received in revised form 5 October 2009; accepted 26 October 2009. published online 19 November 2009.

Abstract

Aims and methods

Apelin is a novel adipokine identified as an endogenous ligand of the G protein-coupled receptor APJ. In this study we compared plasma apelin concentrations in 101 patients with gestational diabetes (GDM) and 101 women with normal glucose tolerance (NGT) between 24 and 32 weeks of gestation (Group 1), as well as in 20 women with GDM and 16 subjects with NGT at term (Group 2). Apelin and APJ mRNA expression in subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT) and placental tissue were also measured in Group 2, using quantitative real-time PCR.

Results

There were no significant differences in plasma apelin levels between the women with GDM and NGT (Group 1: 1555.6 [1281.2–1804.2]pg/ml vs 1656.5 [1430.2–1852.1]pg/ml, Group 2: 1607.9 [1453.4–1768.7]pg/ml vs 1493.8 [1316.8–1956.7]pg/ml) nor in apelin and APJ mRNA expression in SAT, VAT and placental tissue. Apelin mRNA expression was approximately 10 fold higher in placental than in adipose tissue (p<0.0001). Apelin and APJ mRNA expression correlated significantly in SAT (R=0.45, p=0.03), VAT (R=0.69, p=0.003) and placental tissue (R=0.37, p=0.03).