Diabetes Research and Clinical Practice
Volume 86, Issue 3 , Pages 173-176, December 2009

Role of asymmetric-dimethyl-l-arginine (ADMA) and nitrite/nitrate (NOx) in the pathogenesis of oxidative stress in female subjects with uncomplicated type 1 diabetes mellitus

  • Dario Pitocco

      Affiliations

    • Diabetologia, Catholic University, Rome, Italy
    • These authors equally contributed to this article.
    • Corresponding Author InformationCorresponding author at: Servizio di Diabetologia, Pol. Universitario “A. Gemelli”, L.go A. Gemelli, 00168 Roma, Italy. Tel.: +39 06 30154071; fax: +39 06 3050052.
    • ,
  • Francesco Zaccardi

      Affiliations

    • Diabetologia, Catholic University, Rome, Italy
    • These authors equally contributed to this article.
    • ,
  • Enrico Di Stasio

      Affiliations

    • Department of Biochemistry, Catholic University, Rome, Italy
    • ,
  • Federica Romitelli

      Affiliations

    • Department of Biochemistry, Catholic University, Rome, Italy
    • ,
  • Francesca Martini

      Affiliations

    • Diabetologia, Catholic University, Rome, Italy
    • ,
  • Giovanni Luca Scaglione

      Affiliations

    • Department of Biochemistry, Catholic University, Rome, Italy
    • ,
  • Dorina Speranza

      Affiliations

    • Department of Biochemistry, Catholic University, Rome, Italy
    • ,
  • Stefano Santini

      Affiliations

    • Laboratorio Analisi Cliniche e Microbiologiche, IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo (FG), Italy
    • ,
  • Cecilia Zuppi

      Affiliations

    • Department of Biochemistry, Catholic University, Rome, Italy
    • ,
  • Giovanni Ghirlanda

      Affiliations

    • Diabetologia, Catholic University, Rome, Italy

Received 19 June 2009; received in revised form 7 September 2009; accepted 17 September 2009. published online 16 October 2009.

Abstract 

Aims

To explore the role of asymmetric-dimethyl-l-arginine (ADMA), an endogenous nitric oxide synthetases (NOS) inhibitor, and nitrite/nitrate (NOx) in the pathogenesis of oxidative stress in early stages of type 1 diabetes mellitus.

Methods

We measured in 99 female subjects with uncomplicated type 1 diabetes (duration disease <10 years) and in 44 sex-matched controls (comparable for age, smoking habit, diet and physical activity) plasma levels of NOx, glycosylated haemoglobin (HbA1c), glucose, uric acid, total cholesterol, high density lipoprotein (HDL) cholesterol, triglycerides and serum ADMA.

Results

Type 1 diabetic subjects have higher levels of glycemia, HbA1c, LDL cholesterol and NOx, but lower ADMA and serum uric acid (UA), compared with the control group; no further differences were found. A significant linear and inverse correlation was found between NOx and ADMA levels (R2=0.237, p<0.001).

Conclusions

This study suggests a reduced ADMA inhibition of NOS as possible mechanism involved in the pathogenesis of oxidative stress in female subjects with a short duration and uncomplicated type 1 diabetes.

Keywords: Type 1 diabetes mellitus, Asymmetric-dimethyl-l-arginine (ADMA), Nitrite/nitrate (NOx), Uric acid (UA), Oxidative stress

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PII: S0168-8227(09)00411-2

doi:10.1016/j.diabres.2009.09.019

Diabetes Research and Clinical Practice
Volume 86, Issue 3 , Pages 173-176, December 2009

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