Monocyte-derived macrophages from men and women with Type 2 diabetes mellitus differ in fatty acid composition compared with non-diabetic controls

Senanayake, Saranga; Brownrigg, Leon M.; Panicker, Vijay; Croft, Kevin D.; Joyce, David A.; Steer, James H.; Puddey, Ian B.; Yeap, Bu B.

doi:10.1016/j.diabres.2006.07.009

« Previous Next »Diabetes Research and Clinical Practice
Volume 75, Issue 3 , Pages 292-300, March 2007

Monocyte-derived macrophages from men and women with Type 2 diabetes mellitus differ in fatty acid composition compared with non-diabetic controls☆

Saranga Senanayake
- School of Medicine and Pharmacology, University of Western Australia, Fremantle and Royal Perth Hospitals, Western Australia, Australia
Leon M. Brownrigg
- School of Medicine and Pharmacology, University of Western Australia, Fremantle and Royal Perth Hospitals, Western Australia, Australia
Vijay Panicker
- Department of Endocrinology and Diabetes, Fremantle Hospital, Western Australia, Australia
Kevin D. Croft
- School of Medicine and Pharmacology, University of Western Australia, Fremantle and Royal Perth Hospitals, Western Australia, Australia
David A. Joyce
- School of Medicine and Pharmacology, University of Western Australia, Fremantle and Royal Perth Hospitals, Western Australia, Australia
James H. Steer
- School of Medicine and Pharmacology, University of Western Australia, Fremantle and Royal Perth Hospitals, Western Australia, Australia
Ian B. Puddey
- School of Medicine and Pharmacology, University of Western Australia, Fremantle and Royal Perth Hospitals, Western Australia, Australia
Bu B. Yeap
- School of Medicine and Pharmacology, University of Western Australia, Fremantle and Royal Perth Hospitals, Western Australia, Australia
- Department of Endocrinology and Diabetes, Fremantle Hospital, Western Australia, Australia
- Corresponding author at: School of Medicine and Pharmacology, Level 2, T-Block, Fremantle Hospital, Alma Street, Fremantle, Western Australia 6160, Australia. Tel.: +61 8 9431 3229; fax: +61 8 9431 2977.

Received 8 June 2006; accepted 10 July 2006. published online 16 August 2006.

Abstract

We examined whether macrophages from men and women with Type 2 diabetes mellitus (T2DM) exhibited differences in expression of key genes involved in fatty acid metabolism and in fatty acid composition compared with macrophages from non-diabetic controls. Peripheral blood monocytes from subjects with T2DM (n=9) and non-diabetic controls (n=10) were differentiated into macrophages in 10% autologous serum and normal (5mM) or high (22mM) glucose. Levels of PPARα, PPARγ, LXRα, SCD and ABCA1 mRNAs were similar in macrophages from subjects with T2DM and controls. At 5mM glucose, macrophage stearic acid (C18:0) was 12.6±1.0% of total fatty acids for T2DM compared with 18.1±2.0% for controls (p=0.03). Macrophage linoleic acid (C18:2) was 15.5±0.8% for T2DM and 9.3±2.0% for controls (p=0.005). The ratio of macrophage stearic acid (C18:0)/oleic acid (C18:1) was 0.29 [0.25,0.48] for T2DM versus 0.54 [0.36,0.82] for controls (p=0.04). Compared with non-diabetic controls, macrophages from men and women with T2DM had significantly different fatty acid profiles consistent with increased stearoyl-CoA desaturase (SCD) activity and increased C18:2 accumulation. This pattern of altered macrophage fatty acid composition may be relevant to diabetic atherogenesis.

Keywords: Type 2 diabetes, Macrophages, Gene expression, Fatty acids

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☆ Part of the data contained in this manuscript was presented at the Annual Scientific Meeting of the Australian Atherosclerosis Society, Darwin, 19–22 October 2005.

PII: S0168-8227(06)00302-0

doi:10.1016/j.diabres.2006.07.009

« Previous Next »Diabetes Research and Clinical Practice
Volume 75, Issue 3 , Pages 292-300, March 2007

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