Elevation of monocyte-derived microparticles in patients with diabetic retinopathy

Ogata, Nahoko; Nomura, Shosaku; Shouzu, Akira; Imaizumi, Masahito; Arichi, Miwa; Matsumura, Miyo

doi:10.1016/j.diabres.2006.01.014

« Previous Next »Diabetes Research and Clinical Practice
Volume 73, Issue 3 , Pages 241-248, September 2006

Elevation of monocyte-derived microparticles in patients with diabetic retinopathy

Nahoko Ogata
- Department of Ophthalmology, Kansai Medical University, Fumizono-cho 10-15, Moriguchi, Osaka 570-8507, Japan
- Corresponding author. Tel.: +81 6 6992 1001; fax: +81 6 6993 2222.
Shosaku Nomura
- First Department of Internal Medicine, Kansai Medical University, Moriguchi, Osaka, Japan
Akira Shouzu
- Second Department of Internal Medicine, Kansai Medical University, Moriguchi, Osaka, Japan
Masahito Imaizumi
- Department of Ophthalmology, Kansai Medical University, Fumizono-cho 10-15, Moriguchi, Osaka 570-8507, Japan
Miwa Arichi
- Department of Ophthalmology, Kansai Medical University, Fumizono-cho 10-15, Moriguchi, Osaka 570-8507, Japan
Miyo Matsumura
- Department of Ophthalmology, Kansai Medical University, Fumizono-cho 10-15, Moriguchi, Osaka 570-8507, Japan

Received 29 June 2005; received in revised form 19 January 2006; accepted 30 January 2006. published online 03 April 2006.

Abstract

Diabetic retinopathy is associated with microvascular damage and capillary occlusions which are common features of the microangiopathy in diabetes. Monocyte-derived microparticles (MDMPs) are released from activated monocytes and enhance the procoagulant activity, and also activate adhesion reactions. These are key events in the development of capillary occlusion.

The MDMPs level in the blood, and platelet activation markers (platelet-derived microparticles (PDMPs), CD62P and CD63) were measured by flow cytometry in 72 diabetic patients. The plasma levels of intracellular adhesion molecule-1 (ICAM-1) and P-selectin were analyzed by ELISA. The level of MDMPs was significantly correlated with the levels of PDMPs (r=0.52, P<0.001), CD62P (r=0.37, P=0.001), CD63 (r=0.31 and P=0.007), P-selectin (r=0.38, P=0.001), and ICAM-1 (r=0.31, P=0.009). The MDMPs level increased with the progression of the diabetic retinopathy: 81±14/10⁴platelets (plts) in patients without retinopathy (n=10); 88±8/10⁴plts with mild or moderate non-proliferative diabetic retinopathy (NPDR, n=12); 95±8/10⁴plts with severe NPDR (n=24); and 112±9/10⁴plts with proliferative diabetic retinopathy (PDR) (n=26). The MDMPs level in patients with areas of capillary occlusion (123±10/10⁴plts, n=25) was significantly higher than that in patients without areas of capillary occlusion (84±5/10⁴plts, n=25; P=0.0008). These correlations suggest that increased levels of MDMPs may accelerate the progression of diabetic retinopathy.