Psammomys obesus (sand rat), a new animal model of non-alcoholic fatty liver disease

Maislos, Maximo; Medvedovskv, Vitali; Sztarkier, Ignacio; Yaari, Arieh; Sikuler, Emanuel

doi:10.1016/j.diabres.2005.09.007

« Previous Next »Diabetes Research and Clinical Practice
Volume 72, Issue 1 , Pages 1-5, April 2006

Psammomys obesus (sand rat), a new animal model of non-alcoholic fatty liver disease

Maximo Maislos
- Atherosclerosis and Metabolism Unit, Medicine B, Department of Medicine, Soroka UMC and Ben-Gurion University FOHS, Beer-Sheva, Israel
- Department of Medicine B, Soroka UMC and Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
- Corresponding author. Tel.: +972 86403042; fax: +972 86283286.
Vitali Medvedovskv
- Department of Medicine B, Soroka UMC and Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
Ignacio Sztarkier
- Pathology Institute, Soroka UMC and Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
Arieh Yaari
- Liver Research Laboratory, Soroka UMC and Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
Emanuel Sikuler
- Department of Medicine B, Soroka UMC and Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
- Liver Research Laboratory, Soroka UMC and Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel

Received 24 February 2005; received in revised form 4 April 2005; accepted 6 September 2005. published online 19 October 2005.

Abstract

Background/aims

Non-alcoholic fatty liver disease (NAFLD) is an increasingly recognized condition that includes a spectrum of clinicopathologic conditions ranging from steatotosis to cirrhosis and liver failure. NAFLD is usually associated with features of the metabolic syndrome. No established therapies can be offered to patients with NAFLD.

An appropriate animal model of NAFLD would be of help in understanding the mechanisms of the disease and in testing novel therapeutic modalities. Available animal models, such as ob/ob and db/db mice, are unsatisfactory since they show only partial resemblance to human NAFLD. Psammomys obesus (sand rat) is a well-established model of type-2 diabetes and obesity, which shares most metabolic parameters of the human metabolic syndrome. In the present study, we hypothesized that P. obesus will also show features of non-alcoholic fatty liver disease.

Methods

Experimental and control animals were fed normal rat chow or either chow to which fiber (30% wheat straw) was added for 6–18 weeks. Body weight and capillary glucose were measured regularly. At sacrifice blood samples, liver and epididymal fat were obtained. Histology of the liver was blindly determined by a pathologist.

Results

The experimental group showed increased body weight, liver and abdominal fat pad mass, raised plasma glucose, insulin and lipids. Also, alanine-aminotransferase (189±76IU versus 86±26IU; p<0.0001) was significantly higher in the experimental than the control group. Microscopic examination of liver tissue demonstrated marked macrovesicular fat infiltration in the experimental group while it was histologicaly normal in the control animals (liver fat score 1.7±1.0 and 0.2±0.4; p<0.0001, respectively).

Conclusions

Fed a calorie-rich diet P. obesus develops a syndrome, which shares metabolic, laboratory and histopathologic characteristics compatible with human NAFLD.

Keywords: NAFLD, Animal model, Psammomys obesus, Steatohepatitis