Diabetes Research and Clinical Practice
Volume 70, Issue 2 , Pages 103-109, November 2005

Protective effect of vanadyl sulfate on the pancreas of streptozotocin-induced diabetic rats☆

  • Sema Bolkent

      Affiliations

    • Department of Medical Biology, Cerrahpasa Faculty of Medicine, Istanbul University, 34098-Cerrahpasa, Istanbul, Turkey
    • Corresponding Author InformationCorresponding author. Fax: +90 212 632 00 50.
    • ,
  • Sehnaz Bolkent

      Affiliations

    • Department of Biology, Faculty of Science, Istanbul University, 34459-Vezneciler, Istanbul, Turkey
    • ,
  • Refiye Yanardag

      Affiliations

    • Department of Chemistry, Faculty of Engineering, Istanbul University, 34320-Avcilar, Istanbul, Turkey
    • ,
  • Sevim Tunali

      Affiliations

    • Department of Chemistry, Faculty of Engineering, Istanbul University, 34320-Avcilar, Istanbul, Turkey

Accepted 9 February 2005. published online 07 March 2005.

Abstract 

The aim of this study is to examine from a biochemical and histological perspective, whether vanadium has a protective effect on the pancreas of diabetic rats. Male, 6–6.5 months old, Swiss albino rats were divided into four groups. Group I: control (intact) animals (n=13). Group II: control rats given vanadyl sulfate (n=5). Group III: streptozotocin-induced diabetic animals (n=11). Group IV: streptozotocin-induced diabetic animals given vanadyl sulfate (n=11). Vanadyl sulfate was given by gavage technique to rats in a dose of 100mg/kg daily for 60 days, after experimental animals were made diabetic. On day 60, the pancreas tissue and blood samples were taken from the animals. In the streptozotocin-induced diabetic group, blood glucose levels significantly increased in contrast to the loss of body weight, but vanadyl sulfate in streptozotocin-diabetic rats reduced blood glucose levels and increased both blood glutathione levels and body weight. Tissue sections were immunostained using an insulin antibody. The control group given vanadyl sulfate was no different from the other intact control group considering the insulin immunoreactivity in B cells. In pancreatic islets of the diabetic group, a decrease in the number of immunoreactive B cells was observed in comparison to the control group. On the other hand, pancreatic islets of the diabetic group given vanadyl sulfate showed a higher number of immunoreactive B cells in comparison to the diabetic group. According to the immunohistochemical and biochemical results obtained, it was concluded that vanadyl sulfate can regenerate B cells of endocrine pancreas in experimental diabetes.

Keywords: Pancreas, Streptozotocin, Diabetic rats, Vanadyl sulfate

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☆ This study has been presented in part at Joint meeting of the British Association of Clinical Anatomists and the Spanish Anatomical Society, Barcelona, Spain, July 22–23, 2002. Abstracts published European Journal of Anatomy, July 2002, vol. 6, Supplement 1, p. 40.

PII: S0168-8227(05)00036-7

doi:10.1016/j.diabres.2005.02.003

Diabetes Research and Clinical Practice
Volume 70, Issue 2 , Pages 103-109, November 2005

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