Low lipoprotein lipase mass in preheparin serum of type 2 diabetes mellitus patients and its recovery with insulin therapy

Miyashita, Yoh; Shirai, Kohji; Itoh, Yoshiaki; Sasaki, Hidehisa; Totsuka, Mitsuya; Murano, Takeyoshi; Watanabe, Hitoshi

Next »Diabetes Research and Clinical Practice
Volume 56, Issue 3 , Pages 181-187, June 2002

Low lipoprotein lipase mass in preheparin serum of type 2 diabetes mellitus patients and its recovery with insulin therapy

Yoh Miyashita
- Department of Internal Medicine, Sakura Hospital, School of Medicine, Toho University, 564-1 Shimoshizu, Sakura-City, Chiba 285-0841, Japan
- Clinical Laboratory Medicine, Sakura Hospital, School of Medicine, Toho University, 564-1 Shimoshizu, Sakura-City, Chiba 285-0841, Japan
- Corresponding author. Tel.: +81-43-462-8811; fax: +81-43-489-9770
Kohji Shirai
- Department of Pharmacy, Sakura Hospital, School of Medicine, Toho University, 564-1 Shimoshizu, Sakura-City, Chiba 285-0841, Japan
Yoshiaki Itoh
- Department of Internal Medicine, Sakura Hospital, School of Medicine, Toho University, 564-1 Shimoshizu, Sakura-City, Chiba 285-0841, Japan
Hidehisa Sasaki
- Department of Pharmacy, Sakura Hospital, School of Medicine, Toho University, 564-1 Shimoshizu, Sakura-City, Chiba 285-0841, Japan
Mitsuya Totsuka
- Department of Internal Medicine, Sakura Hospital, School of Medicine, Toho University, 564-1 Shimoshizu, Sakura-City, Chiba 285-0841, Japan
Takeyoshi Murano
- Department of Pharmacy, Sakura Hospital, School of Medicine, Toho University, 564-1 Shimoshizu, Sakura-City, Chiba 285-0841, Japan
Hitoshi Watanabe
- Department of Pharmacy, Sakura Hospital, School of Medicine, Toho University, 564-1 Shimoshizu, Sakura-City, Chiba 285-0841, Japan

Received 29 July 2001; received in revised form 2 October 2001; accepted 26 November 2001.

Abstract

There exists lipoprotein lipase mass in preheparin serum, even though the activity is scarcely found. We studied the preheparin serum lipoprotein lipase mass levels (prehaparin LPL mass) in type 2 diabetes mellitus patients and the effect of insulin therapy on the levels of preheparin LPL mass. In 40 type 2 diabetes mellitus patients, preheparin LPL mass were measured by the sandwich enzyme-linked immunosorbent assay (ELISA), and were compared with those of non-diabetic healthy control. The correlation between preheparin LPL mass and Hemoglobin A_1c (HbA_1c), serum lipids were studied. Preheparin LPL mass were measured before and after insulin therapy. Preheparin LPL mass of type 2 diabetes mellitus patients was significantly lower than that of non-diabetic healthy control. In diabetic patients, preheparin LPL mass were negatively correlated with HbA_1c. Fifteen patients started to take insulin therapy. Preheparin LPL mass increased significanaly at 4th week, when fasting blood glucose decreased. These results suggested that preheparin LPL mass was greatly regulated by insulin action.

Keywords: Preheparin LPL mass, Type 2 diabetes mellitus, Insulin therapy